Intestinal Agglomeration Escherichia coli (EAEC) Nucleic Acid Detection Kit (Fluorescent PCR)|BNCC360689 |BNCC

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Intestinal agglomerative Escherichia coli (EAEC) nucleic acid detection kit (Fluorescent PCR method)

Culture medium	Nutritional gravy medium (English name: NA/NB): beef paste 3.0g, peptone 10.0g,NaCl 5.0g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.0. Sterilization at 121 ℃ for 15min. [Note] Adding 5 mgMnSO4 · H2O to culture Bacillus is beneficial to produce spores.
Subculture procedure	Before use, the kit is dissolved on ice, shaken and mixed evenly, nucleic acid extraction or purification reagents need to be provided by oneself, and the specific use method can be seen in the instruction manual.
Storage conditions	-80 ℃, kept away from light
Safety level	0
application	It is suitable for the auxiliary diagnosis of intestinal agglomeration Escherichia coli (EAEC) infection in water-like feces, suspected contaminated water, food and other samples.
Sharing mode	Public welfare sharing

EAEC nucleic acid detection kit certificate

1. Product information

Sample name: Entero-aggregating Escherichia coli (EAEC) nucleic acid detection kit (fluorescence PCR method)

Sample number: 360689

sample batch: 220316

2. Product features

Description	Packaged in 1.5mL cryovials, containing reaction solution, positive quality control material, and negative quality control material
Traceability	CICC24186 intestinal agglomeration Escherichia coli, ATCC25922 Escherichia coli
Product details	2 tubes of reaction solution, 1 tube of positive quality control material, 1 tube of negative quality control material
Field of application	It is suitable for auxiliary diagnosis of EIEC infection in watery stool, suspected contaminated water, food, etc.
*Note: If you have any questions about the reference material, please contact our center (BNCC) for help before use

3. Instructions

Before use, put the kit on ice to dissolve, shake and mix well, nucleic acid extraction or purification reagents should be prepared by yourself.

4. Notes

1. The kit should be stored at low temperature in the dark;

2. All components in the kit should be thawed naturally before use, mixed well and centrifuged briefly to avoid bubbles;

3. Use disposable tips, disposable gloves and wear special work clothes during the experimental operation;

4. Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross-contamination;

5. All items in the kit should be treated as contaminants and handled in accordance with the 《General Principles of Biosafety for Microbial Biomedical Laboratories》;

5. Preservation and transportation

Long-term storage:-20 ℃, valid for 2 years; Transportation conditions: dry ice transportation.

Henan Engineering Technology Research Center of Industrial Microbial Strain

website: www.bncc.org.cn tel: 400-6699-833

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